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A new method for the robust expression and single-step purification of dCas9 for CRISPR interference/activation (CRISPRi/a) applications

Protein Expression and Purification(2024)10.1016/j.pep.2024.106500Source: DataRank Database

A new method for the robust expression and single-step purification of dCas9 for CRISPR interference/activation (CRISPRi/a) applications is a research paper published in Protein Expression and Purification (2024). On theSindex it has a DataRank of 0.269. It has been cited 5 times.

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0.269DataRank · unranked

Scored on demand from live citation data

5 citations

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›Methodology & internals

datarank_citation_only_1hop_v6· scope data_onlyMethodology

Pipeline:MetadataData-paper checkEnrichmentCitation networkScoring

Enrichment:Funding (3 grants)Impact (FWCI 0.79)OA: closedTopics (32)IDs (PubMed, OpenAlex)

FAIR Checklist

Context only (not used in score)

Findable (1/2)

Has DOI

Accessible (0/2)

Interoperable (0/2)

Reusable (0/3)

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DataRank Breakdown

Base Score 100%Citation Network 0%

Base Score Contribution

0.269

From this paper's citation signal

Citation Network Contribution

Citation network not refreshed for this result

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Authors (7)

Binduma Yadav,Koushik Shah,Raminder Kaur,Diksha Choudhary,Nishtha Sharma

Live enrichment

PubMed:38718989 OpenAlex:https://openalex.org/W4396702491

National Agri-Food Biotechnology Institute

Council of Scientific and Industrial Research, India

Department of Biotechnology, Ministry of Science and Technology, India

FWCI

0.79

Citation Percentile

0.7%

Citation Trend

2025

closedLicense: https://doi.org/10.15223/policy-004

publisherhttps://api.elsevier.com/content/article/PII:S104659282400072X?httpAccept=text/xml

publisherhttps://api.elsevier.com/content/article/PII:S104659282400072X?httpAccept=text/plain

journalhttps://doi.org/10.1016/j.pep.2024.106500

repositoryhttps://pubmed.ncbi.nlm.nih.gov/38718989

Fields of Study

CRISPR and Genetic EngineeringAdvanced biosensing and bioanalysis techniquesRNA and protein synthesis mechanismsCRISPR-Cas SystemsEscherichia coliGene EditingCRISPR-Associated Protein 9Gene ExpressionRecombinant Proteins

MeSH Terms

Gene EditingCRISPR-Associated Protein 9Escherichia coliRecombinant ProteinsGene ExpressionCRISPR-Cas Systems

Keywords

Cas9CRISPRTrans-activating crRNANucleaseGuide RNAGenome editingCRISPR interferenceComputational biologyBiologyDNAGeneRNAGeneticsIon exchangeEMSAhydroxyapatite chromatographyDcas9