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Quantitative yeast-yeast two hybrid for discovery and binding affinity estimation of protein-protein interactions

bioRxiv (Cold Spring Harbor Laboratory)(2020)10.1101/2020.08.12.247874Source: DataRank Database

Quantitative yeast-yeast two hybrid for discovery and binding affinity estimation of protein-protein interactions is a research paper published in bioRxiv (Cold Spring Harbor Laboratory) (2020). On theSindex it has a DataRank of 0. It has been cited 6 times.

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Enrichment:Funding (3 grants)OA: greenTopics (55)IDs (PubMed, OpenAlex)

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Authors (7)

Abigail Blain,John Bowen,Matthew Burroughs,Nikki McArthur ORCID,Stefano Menegatti ORCID

Live enrichment

PubMed:21097893 OpenAlex:https://openalex.org/W3048595523

National Science Foundation

Grant: 1510845

UNS: Bi-cyclic peptides for specific inhibition of intracellular protein-protein interactions

National Science Foundation

Grant: 1511227

UNS:Thermal control of avidity for separation of biologicals

National Institutes of Health

Grant: 5T32GM008776-17

Molecular Biotechnology Training at North Carolina State University

greenLicense: cc-by-nc-nd

repositoryhttps://www.biorxiv.org/content/biorxiv/early/2020/08/13/2020.08.12.247874.full.pdf

publisherhttps://syndication.highwire.org/content/doi/10.1101/2020.08.12.247874

repositoryhttps://doi.org/10.1101/2020.08.12.247874

repositoryhttps://figshare.com/articles/journal_contribution/Quantitative_Yeast_Yeast_Two_Hybrid_for_the_Discovery_and_Binding_Affinity_Estimation_of_Protein_Protein_Interactions/14036516

Fields of Study

Transgenic Plants and ApplicationsUbiquitin and proteasome pathwaysViral Infectious Diseases and Gene Expression in Insects0301 basic medicine03 medical and health sciences0303 health sciences

Keywords

YeastTwo-hybrid screeningAffinitiesProtein–protein interactionTandem affinity purificationSaccharomyces cerevisiaeBiologycDNA libraryComputational biologyPlasma protein bindingBiochemistryComplementary DNACell biologyAffinity chromatographyGeneEnzymeChemical Sciences not elsewhere classifiedyeast surface displayBiophysicsMicrobiologyInorganic ChemistryProtein DomainsGenes, ReporterVirologyTwo-Hybrid System TechniquesGeneticsProtein Interaction MapsSmad3 ProteinqYY 2H systemMolecular Biologytandem WW domainsCancerGene LibraryPharmacologymonovalent bait-prey interactionsK DBinding Affinity Estimationbinding affinitiesInfectious DiseasesqYY 2HMedicineaffinityYAPyeast cell surface fusionsproteinPhysical Sciences not elsewhere classifiedSMADProtein BindingTranscription Factors