Expression of Cloned Genes in <i>E. coli</i> Using IPTG-Inducible Promoters is a research paper published in Cold Spring Harbor Protocols (2021). On theSindex it has a DataRank of 0.745. It has been cited 15 times, with 11 citing works in its 1-hop citation network.
Many Escherichia coli expression vectors make use of the lac operon. In general, the lac operator (lacO) is located downstream from the promoter of the target gene, so that binding of the lac repressor blocks transcription initiation until lactose or the isopropyl-β-d-thiogalactopyranoside (IPTG) analog is added. The protocol given here is intended for use with IPTG-inducible vectors. l-Arabinose-inducible systems derived from the ara operon offer an alternative to expression systems based on the lac operon; guidance for their use is also provided.
FAIR checklist signals are shown for context only and do not affect DataRank scoring.
Base Score Contribution
0.416
From this paper's citation signal
Citation Network Contribution
0.329
From 9 citing papers with measurable signal
Ranked by citation count — the same ordering the engine uses when summing log1p(Cq) over citers.
DataRank blends this paper's own citation count with the influence of the papers that cite it. Here, roughly 56% comes from its base citations and 44% from the citation network (9 citing papers contributed measurable signal).
Citers are pulled from OpenAlex sorted by cited_by_count:descand capped per paper, so when the cap binds we keep the highest-signal references and the score is reproducible across reruns.
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