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Optimized Legionella expression strain for affinity purification of His-tagged membrane proteins eliminates major multimeric contaminant

Microbiology Spectrum(2025)10.1128/spectrum.03222-24Source: DataRank Database

Optimized Legionella expression strain for affinity purification of His-tagged membrane proteins eliminates major multimeric contaminant is a research paper published in Microbiology Spectrum (2025). On theSindex it has a DataRank of 0. It has been cited 1 time.

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Open Access1 citations

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datarank_citation_only_1hop_v6· scope data_onlyMethodology

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Enrichment:Funding (8 grants)Impact (FWCI 0.87)OA: goldTopics (46)IDs (PubMed, PMC, OpenAlex)Annotations (3)

FAIR Checklist

Context only (not used in score)

Findable (1/2)

Has DOI

Accessible (1/2)

Open Access

Interoperable (0/2)

Reusable (0/3)

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Authors (9)

Jonasz B. Patkowski ORCID,Joshua Khorsandi,Wandy L. Beatty ORCID,Shanmugapriya Kannaiah ORCID,Ahmet Hasanovic

Live enrichment

PubMed:40387337 PMC:PMC12211046 OpenAlex:https://openalex.org/W4410498888

National Institutes of Health

Grant: AI146093

NIAID NIH HHS

Grant: R01 AI146093

NCATS NIH HHS

Grant: UL1 TR002345

NIDDK NIH HHS

Grant: P30 DK020579

National Institutes of Health

Grant: 2P30DK020579-36

Diabetes Research Center

National Institutes of Health

Grant: 1UL1TR002345-01

Washington University Institute of Clinical and Translational Sciences

National Institutes of Health

Grant: 5P30CA091842-05

Core--Pharmacology Facility

National Institutes of Health

Grant: 5R01AI146093-03

Structure-function analysis of type IVB secretion systems

FWCI

0.87

Citation Percentile

0.7%

Citation Trend

2026

goldLicense: cc-by

journalhttps://doi.org/10.1128/spectrum.03222-24

GOLDhttps://doi.org/10.1128/spectrum.03222-24

publisherhttps://doi.org/10.1128/spectrum.03222-24

publisherhttps://journals.asm.org/doi/pdf/10.1128/spectrum.03222-24

repositoryhttps://pubmed.ncbi.nlm.nih.gov/40387337

Fields of Study

Legionella and Acanthamoeba researchBacterial biofilms and quorum sensingVibrio bacteria research studiesMedicineBiologyChemistry0301 basic medicine03 medical and health sciencesLegionella pneumophilaBacterial ProteinsChromatography, AffinityMembrane ProteinsHistidineEnoyl-CoA Hydratase

MeSH Terms

Bacterial ProteinsChromatography, AffinityEnoyl-CoA HydrataseHistidineMembrane ProteinsLegionella pneumophila

Keywords

Legionella pneumophilaLegionellaStrain (injury)ChemistryMutantMicrobiologyS-layerGeneBacterial outer membraneBiologyBacteriaMolecular biologyEscherichia coliBiochemistryGeneticsProtein purificationSingle particle analysismembrane proteinsCryo-emPolyhistidine Epitope Tagged ProteinsQR1-502Chromatography, AffinityBacterial ProteinsHistidineEnoyl-CoA HydrataseResearch Article