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Optimizing 16S rRNA gene profile analysis from low biomass nasopharyngeal and induced sputum specimens

BMC Microbiology(2020)10.1186/s12866-020-01795-7Source: DataRank Database

Optimizing 16S rRNA gene profile analysis from low biomass nasopharyngeal and induced sputum specimens is a research paper published in BMC Microbiology (2020). On theSindex it has a DataRank of 0. It has been cited 36 times.

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Enrichment:Funding (5 grants)Impact (FWCI 1.27)OA: goldTopics (76)Linked Datasets (25)DataCite Reuse (25)IDs (PubMed, PMC, OpenAlex)Annotations (2)SDGs (1)

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Context only (not used in score)

Findable (2/2)

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Accessible (1/2)

Open Access

Interoperable (2/2)

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Reusable (0/3)

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Authors (6)

Sugnet Gardner‐Lubbe ORCID,Kilaza Samson Mwaikono ORCID,Elloise du Toit ORCID,Heather J. Zar ORCID,Mark P. Nicol ORCID

Live enrichment

PubMed:32397992 PMC:PMC7218582 OpenAlex:https://openalex.org/W3026026036

Bill and Melinda Gates Foundation

Grant: OPP1017641

NHGRI NIH HHS

Grant: U54 HG009824

Foundation for the National Institutes of Health

Grant: 1U01AI110466-01A1

The nasopharyngeal microbiome and respiratory disease in African children

Bill and Melinda Gates Foundation

Grant: OPP1017579

NIAID NIH HHS

Grant: U01 AI110466

FWCI

1.27

Citation Percentile

0.8%

Citation Trend

2020

2021

2022

2023

2024

2025

2026

goldLicense: cc-by

journalhttps://bmcmicrobiol.biomedcentral.com/track/pdf/10.1186/s12866-020-01795-7

publisherhttps://bmcmicrobiol.biomedcentral.com/track/pdf/10.1186/s12866-020-01795-7

journalhttps://doi.org/10.1186/s12866-020-01795-7

repositoryhttps://research-repository.uwa.edu.au/en/publications/47b7937f-d855-43d6-84ee-dacf796c24b2

repositoryhttps://pubmed.ncbi.nlm.nih.gov/32397992

Fields of Study

Molecular Biology Techniques and ApplicationsGenomics and Phylogenetic StudiesFecal contamination and water quality0301 basic medicine03 medical and health sciences0303 health sciencesBacteriaBiomassComputer SimulationDNA, BacterialDNA, RibosomalGene DosageHigh-Throughput Nucleotide SequencingHumansInfant, NewbornNasopharynxRNA, Ribosomal, 16SSequence Analysis, DNASpecimen HandlingSputum

MeSH Terms

BacteriaComputer SimulationDNA, BacterialDNA, RibosomalHumansInfant, NewbornNasopharynxRNA, Ribosomal, 16SSpecimen HandlingSputumSequence Analysis, DNABiomassGene DosageHigh-Throughput Nucleotide Sequencing

Keywords

Biology16S ribosomal RNADNA extractionRibosomal RNA18S ribosomal RNAPolymerase chain reactionGeneMicrobiologyGeneticsOptimizationContaminationReproducibility16S rRNA geneRespiratoryHigh-throughput SequencingLow BiomassBacteriomeNegative ControlsMock ControlsDNA, Bacterial570Gene Dosage610DNA, RibosomalSpecimen HandlingRibonucleic acidGene sequincingNasopharynxRNA, Ribosomal, 16SHumansComputer SimulationBiomassBiomass -- BacteriologyBacteriaBacteria -- IdentificationMethodology ArticleLow biomass nasopharyngeal -- AnalysisInfant, NewbornSputumHigh-Throughput Nucleotide SequencingSequence Analysis, DNAQR1-502

Sustainable Development Goals

SDG 3: 3. Good health

Additional file 5 of Optimizing 16S rRNA gene profile analysis from low biomass nasopharyngeal and induced sputum specimens

10.6084/m9.figshare.12292046figshare

Additional file 5 of Optimizing 16S rRNA gene profile analysis from low biomass nasopharyngeal and induced sputum specimens

10.6084/m9.figshare.12292046.v1figshare

Additional file 6 of Optimizing 16S rRNA gene profile analysis from low biomass nasopharyngeal and induced sputum specimens

10.6084/m9.figshare.12292049.v1figshare

Additional file 6 of Optimizing 16S rRNA gene profile analysis from low biomass nasopharyngeal and induced sputum specimens

10.6084/m9.figshare.12292049figshare

Additional file 1 of ddPCR allows 16S rRNA gene amplicon sequencing of very small DNA amounts from low-biomass samples

10.6084/m9.figshare.17284326.v1figshare

Additional file 1 of ddPCR allows 16S rRNA gene amplicon sequencing of very small DNA amounts from low-biomass samples

10.6084/m9.figshare.17284326figshare

Additional file 2 of ddPCR allows 16S rRNA gene amplicon sequencing of very small DNA amounts from low-biomass samples

10.6084/m9.figshare.17284329.v1figshare

Additional file 2 of ddPCR allows 16S rRNA gene amplicon sequencing of very small DNA amounts from low-biomass samples

10.6084/m9.figshare.17284329figshare

Additional file 1 of Succession and determinants of the early life nasopharyngeal microbiota in a South African birth cohort

10.6084/m9.figshare.26594033.v1figshare

Additional file 1 of Succession and determinants of the early life nasopharyngeal microbiota in a South African birth cohort

10.6084/m9.figshare.26594033figshare

Additional file 1 of Optimizing 16S rRNA gene profile analysis from low biomass nasopharyngeal and induced sputum specimens

10.6084/m9.figshare.12292034.v1figshare

Additional file 10 of Optimizing 16S rRNA gene profile analysis from low biomass nasopharyngeal and induced sputum specimens

10.6084/m9.figshare.12292031.v1figshare

Additional file 1 of Optimizing 16S rRNA gene profile analysis from low biomass nasopharyngeal and induced sputum specimens

10.6084/m9.figshare.12292034figshare

Additional file 2 of Optimizing 16S rRNA gene profile analysis from low biomass nasopharyngeal and induced sputum specimens

10.6084/m9.figshare.12292037.v1figshare

Additional file 10 of Optimizing 16S rRNA gene profile analysis from low biomass nasopharyngeal and induced sputum specimens

10.6084/m9.figshare.12292031figshare

Additional file 2 of Optimizing 16S rRNA gene profile analysis from low biomass nasopharyngeal and induced sputum specimens

10.6084/m9.figshare.12292037figshare

Additional file 3 of Optimizing 16S rRNA gene profile analysis from low biomass nasopharyngeal and induced sputum specimens

10.6084/m9.figshare.12292040.v1figshare

Additional file 3 of Optimizing 16S rRNA gene profile analysis from low biomass nasopharyngeal and induced sputum specimens

10.6084/m9.figshare.12292040figshare

Additional file 4 of Optimizing 16S rRNA gene profile analysis from low biomass nasopharyngeal and induced sputum specimens

10.6084/m9.figshare.12292043.v1figshare

Additional file 7 of Optimizing 16S rRNA gene profile analysis from low biomass nasopharyngeal and induced sputum specimens

10.6084/m9.figshare.12292052.v1figshare

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Optimizing 16S rRNA gene profile analysis from low biomass nasopharyngeal and induced sputum specimens

FAIR Checklist

Authors (6)

Funding (5 grants)

Impact

Open Access

Topics

Linked Datasets (25)

Annotations (Europe PMC)

Related Papers (7)