Microtubule organization in the final stages of cytokinesis as revealed by cryo-electron tomography
Microtubule organization in the final stages of cytokinesis as revealed by cryo-electron tomography is a research paper published in Journal of Cell Science (2011). On theSindex it has a DataRank of 2.0. It has been cited 41 times, with 35 citing works in its 1-hop citation network. Its calibrated FAIR score is 49/100.
Abstract
The completion of cytokinesis is dominated by the midbody, a tightly-packed microtubule (MT)-based bridge that transiently connects the two daughter cells. Assembled from condensed, spindle-MTs and numerous associated proteins, the midbody gradually narrows down until daughter cell partitioning occurs at this site. Although described many years ago, detailed understanding of the abscission process remains lacking. Applying cryo-electron tomography to purified midbodies, in combination with fluorescence microscopy, we present here new insight into MT organization within the midbody. We find that the midbody is spatially divided into a core bundle of MTs that traverses the electron-dense overlap region (continuous MTs), surrounded by MTs that terminate within the overlap region (polar MTs). Residual continuous MTs remained intact up to the verge of abscission, whereas the residual polar MTs lost their organization and retreated from the overlap region at late cytokinesis stages. A detailed localization of the microtubule-bundling protein PRC1 supports the above notion. Our study thus provides a detailed account of the abscission process and suggests that the midbody, having acquired a distinct MT architecture as compared to the preceding central spindle, actively facilitates the final stage of cytokinesis.
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FAIR Checklist
Context only (not used in score)- Has DOI
FAIR checklist signals are shown for context only and do not affect DataRank scoring.
Calibrated FAIR score — a parallel quality metric, independent of the DataRank citation score. See the full evaluation →
DataRank Breakdown
Base Score Contribution
0.561
From this paper's citation signal
Citation Network Contribution
1.5
From 34 citing papers with measurable signal
Top 4 citers driving the network score
Ranked by citation count — the same ordering the engine uses when summing log1p(Cq) over citers.
- Dissection of the Mammalian Midbody Proteome Reveals Conserved Cytokinesis MechanismsScience2004511 citationsDataRank 13.2
- ESCRT Machinery and Cytokinesis: the Road to Daughter Cell SeparationTraffic201192 citationsDataRank 3.9
- Augmin shapes the anaphase spindle for efficient cytokinetic furrow ingression and abscissionMolecular Biology of the Cell201625 citationsDataRank 0.945
- Assembly and breakdown of microtubules within the midbodyCommunicative & Integrative Biology20112 citationsDataRank 0.231
Why this DataRank?
DataRank blends this paper's own citation count with the influence of the papers that cite it. Here, roughly 28% comes from its base citations and 72% from the citation network (34 citing papers contributed measurable signal).
- Base score B(p)
- log1p(citation_count) — grows sub-linearly, so a paper with 1,000 citations is not 10× a paper with 100.
- Network N(p)
- Σ over citers of log1p(Cq) ÷ max(outdegreeq, 1). Being cited by a highly-cited paper with few references counts most.
- Damping factor d = 0.85
- DataRank = (1−d)·B(p) + d·N(p) — the two cards above are each already multiplied by their share.
- Self-citations excluded
- Citers sharing any OpenAlex author ID with this paper are filtered out before the network sum.
Citers are pulled from OpenAlex sorted by cited_by_count:descand capped per paper, so when the cap binds we keep the highest-signal references and the score is reproducible across reruns.
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