Molecular Evidence for Cleavage of Intradimer Phosphodiester Linkage as a Novel Step in Excision Repair of Cyclobutyl Pyrimidine Photodimers in Cultured Human Cells is a research paper published in Journal of Cell Science (1987). On theSindex it has a DataRank of 1.4. It has been cited 21 times, with 19 citing works in its 1-hop citation network.
ABSTRACT A re-analysis of the metabolic fate of ultraviolet light (u.v.)-induced cyclobutyl pyrimidine dimers in the DNA of dermal fibroblasts from patients with different genetic forms of xeroderma pigmentosum (XP), a rare cancer-prone skin disorder, has provided new insight into the mode of dimer repair in normal human cells. When DNA isolated from post-u.v. incubated cultures was subjected to enzymic photoreactivation (PR) to probe dimer authenticity, single-strand scissions were produced in the damaged DNA of incubated XP group A and D cells, but not in DNA from XP group C cells or normal controls. Since enzymic PR treatment ruptures only the cyclobutane ring, these results suggested that in dimer excision-defective XP group A and D strains, the intradimer phosphodiester bond may have been cleaved without site restoration. Such a cleavage event had not previously been detected; the possibility that this reaction may be an early step in the normal excision-repair process is supported by the observed release of free thymidine (dThd) and its monophosphate (TMP), but not of thymine, upon photochemical reversal of the dimercontaining excision fragments isolated from post-u.v. incubated normal cells. The combined number of dThd and TMP molecules released was equal to ≈ 80% of the number of dimers photoreversed; for such release to occur, the dimer must both be at one end of an excised fragment and contain an internal phosphodiester break. Taken together, these data lead us to propose a novel model for dimer repair in human cells in which hydrolysis of the intradimer phosphodiester linkage precedes the concerted action of a generalized ‘bulky lesion-repair complex’ involving conventional strand incision/lesion excision/repair resynthesis/strand ligation reactions.
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Base Score Contribution
0.464
From this paper's citation signal
Citation Network Contribution
0.914
From 19 citing papers with measurable signal
Ranked by citation count — the same ordering the engine uses when summing log1p(Cq) over citers.
DataRank blends this paper's own citation count with the influence of the papers that cite it. Here, roughly 34% comes from its base citations and 66% from the citation network (19 citing papers contributed measurable signal).
Citers are pulled from OpenAlex sorted by cited_by_count:descand capped per paper, so when the cap binds we keep the highest-signal references and the score is reproducible across reruns.
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