Development of A Continuous Fluorescence-Based Assay for N-Terminal Acetyltransferase D
Development of A Continuous Fluorescence-Based Assay for N-Terminal Acetyltransferase D is a research paper published in International Journal of Molecular Sciences (2021). On theSindex it has a DataRank of 0.675. It has been cited 17 times, with 12 citing works in its 1-hop citation network.
Abstract
N-terminal acetylation catalyzed by N-terminal acetyltransferases (NATs) has various biological functions in protein regulation. N-terminal acetyltransferase D (NatD) is one of the most specific NAT with only histone H4 and H2A proteins as the known substrates. Dysregulation of NatD has been implicated in colorectal and lung cancer progression, implying its therapeutic potential in cancers. However, there is no reported inhibitor for NatD yet. To facilitate the discovery of small-molecule NatD inhibitors, we report the development of a fluorescence-based acetyltransferase assay in 384-well high-throughput screening (HTS) format through monitoring the formation of coenzyme A. The fluorescent signal is generated from the adduct in the reaction between coenzyme A and fluorescent probe ThioGlo4. The assay exhibited a Z′-factor of 0.77 and a coefficient of variation of 6%, indicating it is a robust assay for HTS. A pilot screen of 1280 pharmacologically active compounds and subsequent validation identified two hits, confirming the application of this fluorescence assay in HTS.
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FAIR Checklist
Context only (not used in score)- Has DOI
FAIR checklist signals are shown for context only and do not affect DataRank scoring.
DataRank Breakdown
Base Score Contribution
0.434
From this paper's citation signal
Citation Network Contribution
0.241
From 9 citing papers with measurable signal
Top 4 citers driving the network score
Ranked by citation count — the same ordering the engine uses when summing log1p(Cq) over citers.
- Tissue sulfhydryl groupsArchives of Biochemistry and Biophysics195926,079 citationsDataRank 1.5
- A Simple Statistical Parameter for Use in Evaluation and Validation of High Throughput Screening AssaysSLAS Discovery19996,921 citationsDataRank 1.3
- Application of reverse-phase HPLC to quantify oligopeptide acetylation eliminates interference from unspecific acetyl CoA hydrolysisBMC Proceedings200921 citationsDataRank 1.5
- Using cell lysates to assess N-terminal acetyltransferase activity and impairmentMethods in Enzymology20232 citationsDataRank 0.190
Why this DataRank?
DataRank blends this paper's own citation count with the influence of the papers that cite it. Here, roughly 64% comes from its base citations and 36% from the citation network (9 citing papers contributed measurable signal).
- Base score B(p)
- log1p(citation_count) — grows sub-linearly, so a paper with 1,000 citations is not 10× a paper with 100.
- Network N(p)
- Σ over citers of log1p(Cq) ÷ max(outdegreeq, 1). Being cited by a highly-cited paper with few references counts most.
- Damping factor d = 0.85
- DataRank = (1−d)·B(p) + d·N(p) — the two cards above are each already multiplied by their share.
- Self-citations excluded
- Citers sharing any OpenAlex author ID with this paper are filtered out before the network sum.
Citers are pulled from OpenAlex sorted by cited_by_count:descand capped per paper, so when the cap binds we keep the highest-signal references and the score is reproducible across reruns.
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