Improved comparative proteome analysis based on two‐dimensional gel electrophoresis is a research paper published in PROTEOMICS (2007). On theSindex it has a DataRank of 1.9. It has been cited 38 times, with 31 citing works in its 1-hop citation network.
AbstractThe purpose of this study was to test the extent to which differences in spot intensity can be reliably recognized between two groups of two‐dimensional electrophoresis gels (pH 4–7, visualized with ruthenium fluorescent stain) each loaded with different amounts of protein from rat brain (power analysis). Initial experiments yielded only unsatisfactory results: 546 spots were matched from two groups of 6 gels each loaded with 200 µg and 250 µg protein, respectively. Only 72 spots were higher (p<0.05), while 58 spots were significantly lower in the 250‐µg group. The construction of new apparatuses that allowed the simultaneous processing of 24 gels throughout all steps between rehydration and staining procedure considerably lowered the between‐gel variation. This resulted in the detection of significant differences in spot intensities in 77–90% of all matched spots on gel groups with a 25% difference in protein load. This applied both when protein from 24 biological replicates was loaded onto two groups of 12 gels and when two pooled tissue samples were each loaded onto 6 gels. At a difference of 50% in protein load, more than 90% of all spots differed significantly between two experimental groups.
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Base Score Contribution
0.550
From this paper's citation signal
Citation Network Contribution
1.3
From 24 citing papers with measurable signal
Ranked by citation count — the same ordering the engine uses when summing log1p(Cq) over citers.
DataRank blends this paper's own citation count with the influence of the papers that cite it. Here, roughly 29% comes from its base citations and 71% from the citation network (24 citing papers contributed measurable signal).
Citers are pulled from OpenAlex sorted by cited_by_count:descand capped per paper, so when the cap binds we keep the highest-signal references and the score is reproducible across reruns.
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