Recombinant Heptameric Coatomer Complexes: Novel Tools to Study Isoform‐Specific Functions is a research paper published in Traffic (2011). On theSindex it has a DataRank of 0.829. It has been cited 28 times, with 11 citing works in its 1-hop citation network.
COPI (coat protein I)‐coated vesicles are implicated in various transport steps within the early secretory pathway. The major structural component of the COPI coat is the heptameric complex coatomer (CM). Recently, four isoforms of CM were discovered that may help explain various transport steps in which the complex has been reported to be involved. Biochemical studies of COPI vesicles currently use CM purified from animal tissue or cultured cells, a mixture of the isoforms, impeding functional and structural studies of individual complexes. Here we report the cloning into single baculoviruses of all CM subunits including their isoforms and their combination for expression of heptameric CM isoforms in insect cells. We show that all four isoforms of recombinant CM are fully functional in an in vitro COPI vesicle biogenesis assay. These novel tools enable functional and structural studies on CM isoforms and their subcomplexes and allow studying mutants of CM.
FAIR checklist signals are shown for context only and do not affect DataRank scoring.
Base Score Contribution
0.505
From this paper's citation signal
Citation Network Contribution
0.324
From 10 citing papers with measurable signal
Ranked by citation count — the same ordering the engine uses when summing log1p(Cq) over citers.
DataRank blends this paper's own citation count with the influence of the papers that cite it. Here, roughly 61% comes from its base citations and 39% from the citation network (10 citing papers contributed measurable signal).
Citers are pulled from OpenAlex sorted by cited_by_count:descand capped per paper, so when the cap binds we keep the highest-signal references and the score is reproducible across reruns.
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