<i>Pseudomonas aeruginosa lasR</i> mutant fitness in microoxia is supported by an Anr-regulated oxygen-binding hemerythrin is a research paper published in Proceedings of the National Academy of Sciences (2020). On theSindex it has a DataRank of 1.4. It has been cited 69 times, with 47 citing works in its 1-hop citation network.
Pseudomonas aeruginosa strains with loss-of-function mutations in the transcription factor LasR are frequently encountered in the clinic and the environment. Among the characteristics common to LasR-defective (LasR-) strains is increased activity of the transcription factor Anr, relative to their LasR+ counterparts, in low-oxygen conditions. One of the Anr-regulated genes found to be highly induced in LasR- strains was PA14_42860 (PA1673), which we named mhr for microoxic hemerythrin. Purified P. aeruginosa Mhr protein contained the predicted di-iron center and bound molecular oxygen with an apparent Kd of ∼1 µM. Both Anr and Mhr were necessary for fitness in lasR+ and lasR mutant strains in colony biofilms grown in microoxic conditions, and the effects were more striking in the lasR mutant. Among genes in the Anr regulon, mhr was most closely coregulated with the Anr-controlled high-affinity cytochrome c oxidase genes. In the absence of high-affinity cytochrome c oxidases, deletion of mhr no longer caused a fitness disadvantage, suggesting that Mhr works in concert with microoxic respiration. We demonstrate that Anr and Mhr contribute to LasR- strain fitness even in biofilms grown in normoxic conditions. Furthermore, metabolomics data indicate that, in a lasR mutant, expression of Anr-regulated mhr leads to differences in metabolism in cells grown on lysogeny broth or artificial sputum medium. We propose that increased Anr activity leads to higher levels of the oxygen-binding protein Mhr, which confers an advantage to lasR mutants in microoxic conditions.
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Base Score Contribution
0.637
From this paper's citation signal
Citation Network Contribution
0.812
From 33 citing papers with measurable signal
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DataRank blends this paper's own citation count with the influence of the papers that cite it. Here, roughly 44% comes from its base citations and 56% from the citation network (33 citing papers contributed measurable signal).
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