A downstream CA repeat sequence increases translation from leadered and unleadered mRNA in <i>Escherichia coli</i> is a research paper published in Molecular Microbiology (1999). On theSindex it has a DataRank of 3.7. It has been cited 74 times, with 64 citing works in its 1-hop citation network.
When placed downstream of the start codon, multimers of the dinucleotide CA stimulated translation from lacZ, gusA and neo mRNAs in the presence or absence of an untranslated leader sequence. Enhanced expression in the absence of a leader and Shine–Dalgarno sequence indicated that stimulation by CA multimers was independent of translation signals contained within the untranslated leader. Multimers of CA stimulated a significantly higher level of lacZ expression than multimers of individual C or A nucleotides. Translation levels increased as the number of CA repeats increased; fewer multimers were required for enhanced expression from leadered mRNA than from mRNA that was deleted for its leader sequence. Addition of downstream CA multimers increased the ribosome binding strength of mRNA in vitro and the amount of full‐length mRNA in vivo, suggesting that the enhanced expression resulted from translation of a more abundant functional message containing a stronger ribosome binding site. The presence of downstream CA‐rich sequences, occurring naturally in several Escherichia coli genes, might contribute to translation of other mRNAs. Addition of CA multimers might represent a general mechanism for increasing expression from genes of interest.
FAIR checklist signals are shown for context only and do not affect DataRank scoring.
Base Score Contribution
0.648
From this paper's citation signal
Citation Network Contribution
3.1
From 57 citing papers with measurable signal
Ranked by citation count — the same ordering the engine uses when summing log1p(Cq) over citers.
DataRank blends this paper's own citation count with the influence of the papers that cite it. Here, roughly 17% comes from its base citations and 83% from the citation network (57 citing papers contributed measurable signal).
Citers are pulled from OpenAlex sorted by cited_by_count:descand capped per paper, so when the cap binds we keep the highest-signal references and the score is reproducible across reruns.
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