The Initiation Codon Affects Ribosome Binding and Translational Efficiency in <i>Escherichia coli</i> of <i>c</i> I mRNA with or without the 5′ Untranslated Leader is a research paper published in Journal of Bacteriology (2001). On theSindex it has a DataRank of 4.6. It has been cited 98 times, with 91 citing works in its 1-hop citation network.
ABSTRACT Translational efficiency of an AUG, CUG, GUG, or UUG initiation codon was measured for the naturally leaderless c I mRNA from bacteriophage λ. In a c I- lacZ translational fusion, only AUG supported a high level of expression; GUG supported a low level of expression, while UUG and CUG expression was barely above background levels. Addition of an untranslated lac leader and Shine-Dalgarno sequence to c I increased expression but still showed a dependence on an AUG for maximum expression. c I- lacZ mRNA with an AUG initiation codon showed a greater in vitro ribosome binding strength and a higher level of full-length in vivo mRNA, suggesting that the initiation codon is an important determinant of ribosome binding strength and translational efficiency for mRNA with or without the 5′ untranslated leader.
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Base Score Contribution
0.689
From this paper's citation signal
Citation Network Contribution
3.9
From 80 citing papers with measurable signal
Ranked by citation count — the same ordering the engine uses when summing log1p(Cq) over citers.
DataRank blends this paper's own citation count with the influence of the papers that cite it. Here, roughly 15% comes from its base citations and 85% from the citation network (80 citing papers contributed measurable signal).
Citers are pulled from OpenAlex sorted by cited_by_count:descand capped per paper, so when the cap binds we keep the highest-signal references and the score is reproducible across reruns.
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